CESIUM CHLORIDE GRADIENT CENTRIFUGATION Meaning and
Definition
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Cesium chloride gradient centrifugation is a laboratory technique commonly used in biochemistry and molecular biology to separate and purify biological molecules such as nucleic acids or proteins based on their density.
This technique utilizes a dense salt solution called cesium chloride (CsCl) to create a density gradient within a centrifuge tube. The gradient is typically formed by layering CsCl solutions of different concentrations on top of each other. As the sample is loaded onto the gradient, it settles and separates into distinct bands according to its density.
The centrifuge tube containing the sample is then placed in a centrifuge machine, which applies centrifugal force to cause the molecules to migrate along the gradient. Over time, the particles will move through the gradient until they reach a position where the density of the solution matches their own density. At this point, the particles become buoyant and remain in a specific location within the gradient, forming a distinct band.
Cesium chloride gradient centrifugation is particularly useful for isolating nucleic acids, such as DNA or RNA, from complex mixtures. The technique allows for their separation from other cellular components, such as proteins or polysaccharides, based on differences in their buoyant densities. These isolated nucleic acids can then be further analyzed or used for various experimental purposes.
Overall, cesium chloride gradient centrifugation is an important tool in molecular biology research, providing a method for the purification and segregation of biologically significant molecules based on their density.
Common Misspellings for CESIUM CHLORIDE GRADIENT CENTRIFUGATION
- xesium chloride gradient centrifugation
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- cwsium chloride gradient centrifugation
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- cewium chloride gradient centrifugation
- cesuum chloride gradient centrifugation
- cesjum chloride gradient centrifugation
- ceskum chloride gradient centrifugation
- cesoum chloride gradient centrifugation
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